Antidiabetic and Hypolipidemic Properties of Newly Isolated Wild Lacticaseibacillus paracasei Strains in Mature Adipocytes

This study investigates the antidiabetic and hypolipidemic potential of newly isolated Lacticaseibacillus paracasei strains in mature adipocytes. Differentiated 3T3-L1 cells are treated with 10% cell-free supernatants (CFSs) from four autochthonous (wild) (M2.1, C8, C15, P4) paracasei. Glucose consumption, intracellular lipid deposition, lipolysis rates, some gene expressions related to adipocyte insulin sensitivity evaluated. The results show that all CFS-treated groups experienced a substantial increase glucose uptake, indicating promising for countering glucotoxicity resistance. different had notable differences metabolic pathway modulation. Generally, P4 CFS supplementation seems enhance insulin-dependent inflow, while M2.1, C15 stimulate insulin-independent consumption by M2.1 CFSs ameliorate buffer capacity enhancing accumulation reducing rate—an advantageous therapeutic effect overweight individuals subjected obesity-predisposing factors. Notably, C8 suppressed expression crucial markers, an unfavorable outcome risk prolonged treatment. Overall, our findings suggest may be implemented as nutraceuticals counteract resistance, potentially easing health status obese individuals.